nohup platanus assemble -o Larrrea -f ../unknown_NoIndex_L000_R1.fastq ../unknown_NoIndex_L000_R2.fastq -t 10 -m 150 &
-o 输出文件头的名字
-f 你的序列文件
-t 线程数
-m 最大内存数
platanus assemble/scaffold/gap_close
Platanus version: 1.2.1
Usage platanus assemble [Options]
Options:
-o STR : prefix of output files (default out, length <= 200)
-f FILE1 [FILE2 ...] : reads file (fasta or fastq, number <= 100)
-k INT : initial k-mer size (default 32)
-s INT : step size of k-mer extension (>= 1, default 10)
-n INT : initial k-mer coverage cutoff (default 0, 0 means auto)
-c INT : minimun k-mer coverage (default 2)
-a FLOAT : k-mer extension safety level (default 10.0)
-u FLOAT : maximum difference for bubble crush (identity, default 0.1)
-d FLOAT : maximum difference for branch cutting (coverage ratio, default 0.5)
-t INT : number of threads (<= 100, default 1)
-m INT : memory limit for making kmer distribution (GB, >=1, default 16)
Outputs:
PREFIX_contig.fa
PREFIX_contigBubble.fa
PREFIX_kmerFrq.tsv
freemao
FAFU