单细胞测序 RNA velocity | RNA速率

RNA velocity：the time derivative of the gene expression state—can be directly estimated by distinguishing between unspliced and spliced mRNAs in common single-cell RNA sequencing protocols. a high-dimensional vector that predicts the future state of individual cells on a timescale of hours.

half-life of mRNA：Translation in both prokaryotes and eukaryotes involves three phases: initiation, elongation, and termination. The relative abundance of nascent (unspliced) and mature (spliced) mRNA can be exploited to estimate the rates of gene splicing and degradation. could reveal the rate and direction of change of the entire transcriptome during dynamic processes.

RNA的速率——基因表达状态的时间导数——可以通过区分普通单细胞RNA测序中未剪接和剪接的mrna来直接估计。

 

 

 

REF
RNA velocity of single cells
https://www.nature.com/articles/s41586-018-0414-6
https://www.cnblogs.com/leezx/tag/单细胞/
https://www.cnblogs.com/leezx/p/9504896.html
https://www.bilibili.com/video/av288296787/