1.NCBI文件格式如下:
2.格式转换
(1) bas.h5 -> ccs
source /share/nas2/genome/biosoft/smrtanalysis/2.3.0/smrtanalysis/current/etc/setup.sh
bash5tools.py --readType subreads --outType fasta /path/to/bas.h5
(2) bax.h5 -> ccs
需要各个文件进行转换并进行合并
source /opt/smrtanalysis/install/smrtanalysis_2.3.0.140936/etc/setup.sh
ConsensusTools.sh CircularConsensus m161112_054131_42199_c101122602550000001823257305221784_s1_p0.3.bax.h5
3.文件格式
PacBio CCS (Circular Consensus Sequence) or RoI (Read of Insert) read
@<MovieName>/<ZMW_number>
(sequence)
PacBio CCS subread
@<MovieName> /<ZMW_number>/<subread-start>_<subread-end>